EFFECT OF FREEZING AND STORAGE TIME OF BLOOD SERUM OF RAT IN THE DETERMINATION OF BIOCHEMICAL PARAMETERS

  • Denise Perone
  • Renata Campos Capela
  • Regina Adriana de Oliveira Calsolari
  • Leandro Alves dos Santos
  • Igor de Carvalho Deprá
  • Corina Julieta Correa Tomasetti
  • Juliana Silva Siqueira Universidade Estadual Paulista Julio de Mesquita Filho (Unesp) - Câmpus Botucatu
  • Patrícia Pintor dos Reis
  • Priscilla Loiola
  • Dijon Henrique Salomé de Campos
  • Camila Renata Correa
Keywords: Temperature, Experimental model, Analytical reliability, Biochemical stability

Abstract

This study holds significant relevance for the experimental research setting, where numerous samples are stored for subsequent biochemical analyses. The aim was to evaluate the stability of biochemical tests in serum from Wistar rats maintained at different temperatures for up to 360 days. The study was conducted at the School of Medicine, São Paulo State University (UNESP), Experimental Research Unit (UNIPEX), Botucatu – SP, Brazil. Serum samples were obtained from 30 male Wistar rats, approximately four months old. Analyses were performed immediately after collection (time zero) and after 30, 180, and 360 days of storage at -20°C and -80°C. The analytes assessed were cholesterol, albumin, urea, total protein, triglycerides, and glucose. The results showed that time and temperature influence some biochemical parameters, except for glucose, which remained stable for 360 days. The findings indicate that extremely low temperatures do not necessarily ensure better preservation of samples.

Published
2026-03-17
Section
Ciências da Saúde